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Linking the ancient group O strain from a 1960 infection to the Y181 group O lineage. An 817-bp fragment (integrase to vif) of group O variants was aligned with the only sequenced region of the Norwegian HIV-1 group O infection dated to 1960 (HIV1T29). A maximum-likelihood phylogenetic tree was constructed using PhyML, as described in more detail in Materials and Methods. The tree was rooted with HXB2 and SIVcpz_US_Marilyn as the out group.
