FIG. 1.

Alignment of US3 kinases. The amino acid sequences of VZV ORF66p, HSV-1 US3 and US3.5, PRV US3a, and US3b were aligned, and positions marked with asterisks identify identical amino acids conserved in all species. Positions marked with dots identify similarities. A Kalign (http://www.ebi.ac.uk/Tools/kalign/index.html) alignment was performed between VZV ORF66p and the cellular kinases PKA and CDK2 to identify 12 kinase subdomains (VIA and VIB are independent domains) conserved within the eukaryotic protein kinase superfamily (25). Vertical red boxes identify invariant residues conserved in all eukaryotic kinases. The black horizontal box identifies the known catalytic loop region of VZV ORF66p.