FIG. 5.

Analysis of HDAC2 hyperphosphorylation in cells infected with wild-type and US3 kinase-deficient viruses. 293A cells were mock infected or infected with wild-type (VZV-WT) or ORF66p kinase-deficient (VZV-ORF66KD) VZV at an MOI of 0.025 or with wild-type (HSV-1 WT) or US3-deleted (HSV-1 US3 null) HSV-1 or wild-type (PRV-WT) or US3-deleted (PRV-US3 null) PRV at an MOI of 0.1. Cells were harvested at between 48 and 96 hpi, when all of the cells were infected as determined by phase microscopy and GFP expression. Twenty micrograms of total protein from each sample was analyzed by Western blotting using antisera specific for HDAC2, VZV gE, EGFP, HSV-1 US3, HSV-1 gB, PRV US3, PRV gC, and HSP90. The percentage of hyperphosphorylated HDAC2 (% Phos) that accumulated during infection with each virus, relative to the total amount of HDAC2, was quantified using ImageJ software.