FIG. 6.

Analysis of HDAC2 in infected cell extracts following incubation with calf intestine alkaline phosphatase. Vero cells were mock infected or infected with, wild-type PRV (PRV-WT), US3 null PRV (PRV-US3 null), wild-type HSV-1 (HSV-1 WT), or US3 null HSV-1 (HSV-1 US3 null) at an MOI of 0.1 and harvested at 48 hpi, when all cells were infected. Lysates from mock- and virus-infected cells were reacted with dephosphorylation buffer alone (mock treated) or calf intestinal alkaline phosphatase (CIAP treated) as described in Materials and Methods. Twenty micrograms of total protein for each sample was analyzed by Western blotting using antisera specific for HDAC2 and HSP90.