FIG. 10.
miR200 family members inhibition via ZEB1 or ZEB2 overexpression decreases EBV lytic gene expression. (A and C) Immunoblots showing relative levels of the indicated proteins in HONEAkata (A) and CNE1Akata (C) cells following transfection with 0, 0.5, or 1.0 μg of an expression plasmid to ZEB1 or ZEB2, as indicated, along with 1.0, 0.5, or 0 μg of their empty vector, pcDNAhismaxC, to apply 1.0 μg total DNA per well in a 6-well plate. Forty-eight hours later, whole-cell extracts were prepared for protein (A and C) and RNA (B and D). The proteins were analyzed for ZEB1, ZEB2, BZLF1, and BMRF1 levels as described in the legend to Fig. 4. (B and D) Relative levels of miRNAs 200a, 200b, and 429 present in the cells from panels A and C, respectively. Levels of miRNAs 200a, 200b, and 429 were determined by RT-qPCR as described in Materials and Methods, with normalization to RNU38B.