Relative luciferase expression levels after cotransfection of FPV-T7-infected QM5 cells with the viral minigenome plasmid (containing the leader and trailer sequences of Herts that flank the Gluc reporter gene) and plasmids expressing NP, P, and L of Herts (gray) or AV324 (black). The background level of luciferase activity was determined by omitting the L plasmid (X). The graph shows the average values obtained in six separate experiments. On the left y axis, values for luciferase activities relative to Herts are given, and on the right y axis, values for luciferase activities relative to AV324 are shown. Error bars represents standard errors of the means (SEMs).