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Enzyme kinetics parameters for clade B and AE-WT and NFV-resistant variants
| Parameter | Result for indicated variant |
|||
|---|---|---|---|---|
| B-WT | B-D30N/N88D | AE-WT | AE-N88S | |
| Km (μM) | 16.7 ± 6.0 | 35.9 ± 0.1 | 17.5 ± 4.0 | 19.0 ± 0.8 |
| kcat (s−1) | 1.79 ± 0.28 | 0.13 ± 0.09 | 0.70 ± 0.08 | 0.20 ± 0.02 |
| kcat/Km (s−1 μM−1) | 0.11 ± 0.04 | 0.004 ± 0.002 | 0.04 ± 0.01 | 0.010 ± 0.001 |
