FIGURE 6.

High frequency of IFN-γ⁺IL-17⁺ double-positive cells is detected in the brain during the course of i.c. BCG infection. A, Representative dot plots show IFN-γ⁺ and IL-17⁺-producing cells from a CD4⁺LFA-1^high T cell gate from the brain and spleen at 3 and 5 weeks post i.c. BCG infection. Numbers represent the frequency of each quadrant among CD4⁺LFA-1^high T cell cells. B, Summary graphs show the proportion of IFN-γ⁻IL-17⁺ and IFN-γ⁺IL-17⁺ cells in CD4⁺LFA-1^highIL-17⁺ T cells from the brain and spleen at 3 and 5 weeks post i.c. BCG infection. Three to six mice were used per group.