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. 2010 Jul 9;285(37):28472–28480. doi: 10.1074/jbc.M110.133520

FIGURE 4.

FIGURE 4.

Curcumin treatment alters the turnover rate of both mature and immature APP in H4-APP751 cells. H4-APP751 cells were treated with 20 μm curcumin for 24 h and then treated with 40 μg/ml cycloheximide for different time (0, 0.5, 1.5, and 3 h). Cell lysates were collected and utilized for Western blotting analysis. Cell lysates were probed with the APP8717 antibody to reveal APP. β-Actin was used as the loading control. A, a representative gel revealing the cycloheximide treatment of different time points with or without curcumin treatment. B and C, quantitative Western blot analysis for mature (B) and immature (C) APP levels. Immature APP levels in curcumin treatment were significantly higher at 0.5, 1.5, and 3 h, compared with the corresponding control treatment. D, quantitative Western blot analysis for total APP levels. E, the ratio of APPma:APPim was decreased in curcumin treatment compared with control at cycloheximide treatment of 0, 0.5, 1.5, and 3 h (n = 3 for each treatment group). Mean ± S.E. *, p < 0.05; **, p < 0.01.