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. 2010 Jul 13;285(37):28496–28505. doi: 10.1074/jbc.M110.131870

FIGURE 8.

FIGURE 8.

Binding of Dna2, Pif1, RPA, and FEN1. Lane 1, a nonspecific IgG control; lane 2, a positive control for 200 pg of purified FEN1. Lanes 3 (containing 1 ng of purified FEN1 and 1 ng of purified Dna2), and 4 (containing 2 ng of purified Pif1, 2 ng of purified RPA, 1 ng of purified FEN1, and 1 ng of purified Dna2) were immunoprecipitated (IP) with antibody to Dna2. Lanes 5 (containing 1 ng of purified FEN1 and 1 ng of purified Pif1), and 6 (containing 2 ng of purified Dna2, 2 ng of purified RPA, 1 ng of purified FEN1, and 1 ng of purified Pif1) were immunoprecipitated with antibody to Pif1. Lanes 7 (containing 1 ng of purified FEN1 and 1 ng of purified RPA) and 8 (containing 2 ng of purified Dna2, 2 ng of purified Pif1, with 1 ng of purified FEN1 and 1 ng of purified RPA) were immunoprecipitated with antibody to RPA. The immunoprecipitates were separated on a SDS-polyacrylamide gel, and Western blotting (IB) was performed using antibody against FEN1.