FIGURE 6.

hCAF1 represses translation independent of deadenylation. A and B, the relative translation of luciferase activity in response to HA:MS2:hCAF1 (A) or HA:MS2:hCAF1 (B) wild type or catalytic mutant was quantified as the ratio of firefly to Renilla luciferase activity. The reporter used in the experiment is indicated. Normalization and error bars were determined as for Fig. 1B. Bottom panel, lysates equivalent to two oocytes were loaded in each lane and analyzed by Western blotting with α-HA-11 and α-actin.