FIGURE 7.

XlCAF1 deadenylation rates and translational repression. A, poly(A) status of reporter RNA. Lanes 1 and 3 show the ³²P RNA without a poly(A) before and after injection into oocytes, respectively. Lanes 2 and 4 show the ³²P RNA with a poly(A) tail before and after injection into oocytes, respectively. Lanes 5 and 6 indicate poly(A) tail length of the ³²P RNA when tethered to the control HA:MS2 fusion protein. Lanes 7–10 and 11–14 indicate the poly(A) tail length of the ³²P RNA when tethered HA:MS2:CAF1a or HA:MS2:b for designated lengths of time, respectively. B, the relative translation of firefly luciferase activity 1 h post injection of the reporter mRNA in response to each protein. The luciferase mRNA contained three MS2-binding sites in the 3′-UTR and a poly(A) tail of 39 adenosines. Normalization and error bars were determined as for Fig. 1B.