FIGURE 5.
The standard Michaelis-Menten law fits the Pels enzymatic activity as a function of the PGA concentration. Assays were performed in minimal medium M63 buffer as described under “Experimental Procedures.” Pel activity was normalized to the maximal Pel activity. The value of Km is classically extracted as the substrate concentration giving half of the maximum velocity. (Here Km = 1.2 g/liter.) Each value represents the mean of three independent experiments. The bars indicate the standard deviation.