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. 2010 Jul 15;285(37):28643–28650. doi: 10.1074/jbc.M110.107649

FIGURE 5.

FIGURE 5.

Binding of the anti-MyoGEF peptide antibody to the C terminus of MyoGEF interferes with the GIPC1-MyoGEF interaction. A, immunoblot analysis of MDA-MB-231 cells transfected with plasmids encoding Myc-tagged wild-type MyoGEF (Myc-WT) or mutant MyoGEF lacking the C-terminal 10 amino acid residues (Myc-ΔC10). WB, Western blot. B, immunoblot analysis of MDA-MB-231 cells transfected with plasmids encoding GFP-WT MyoGEF (GFP-WT) or GFP-MyoGEFΔSEV (GFP-ΔSEV). C, 10 μg of purified GST-MyoGEF-(501–790) was subjected to SDS-PAGE, followed by Coomassie Blue staining. D, in vitro interactions between GST-MyoGEF-(501–790) (GST-501–790) and Myc-GIPC1 in the presence or absence of the anti-MyoGEF peptide antibody (Ab).