FIGURE 4.

polo mad2 double mutant cells exit mitosis. A, third instar larval brains were stained with Hoechst, and the percentage of cells in mitosis was calculated for polo mutants (33%, n = 1777), polo mad2 double mutants (5.8%, n = 3082), and wild type cells (1%, n = 6575). Note that in every case the polo mutants and double mutants used were trans-heterozygous for polo¹⁰ over a deficiency (see supplemental materials). B, third instar larval brains were stained with TUNEL, and the percentage of cells in apoptosis was calculated for polo mutants (18.5%, n = 3415), polo/mad2 double mutants (4.4%, n = 1469), and wild types (0.9%, n = 4267). The number of mitotic and TUNEL-positive cells is shown as the mean with a 95% confidence interval.