FIGURE 4.

Effect of polyamines on the synthesis of RMF-β-Gal fusion proteins derived from wild and mutated rmf-lacZ mRNAs in the 5′-untranslated region. A, possible secondary structure of the initiation region of rmf mRNA was constructed by the method of Zuker (34). The positions in which the sequence was mutated are shown. Symbols of ▵ and → in parenthesis represent removal and replacement of nucleotide, respectively. B, effect of polyamines on RMF-β-Gal fusion protein synthesis was evaluated by Western blot analysis using 5 μg of protein of cell lysate and antibody against β-galactosidase. Values are means ± S.E. of triplicate determinations. ns, p > 0.05; **, p ≤ 0.01.