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Inhibiting autophagy reduced IFN-γ-induced inflammation. Griess reagent was used to detect nitrite generation 48 h after IFN-γ (10 ng/ml) treatment in WT MEFs (A) and RAW264.7 cells (B) pretreated for 0.5 h with (+) and without (−) 3-MA (1 mm). ELISA was used to measure RANTES and TNF-α 24 h after treatment. Dimethyl sulfoxide (DMSO) was the control. Data, obtained from triplicate cultures, are means ± S.D. (error bars). *, p < 0.05.
