FIGURE 4.

Autophagy was important for IFN-γ-activated STAT1. A, after IFN-γ (10 ng/ml) treatment, Western blotting was used to determine the time kinetic phosphorylation of STAT1α/β (Tyr⁷⁰¹) as well as IRF1 and SOCS1 expression in WT and Atg5^−/− MEFs. B, HMEC-1 cells were pretreated with or without lentivirus-based short hairpin Atg5 RNA (shAtg5) or control luciferase-shRNA (shLuc) transfection and then treated with IFN-γ (10 ng/ml) for the indicated times. Western blotting was used to determine the expression of phosphorylation of STAT1α/β (Tyr⁷⁰¹) and Atg5. β-Actin was the internal control. Data are representative of three individual experiments.