FIGURE 5.

Autophagy was required for IFN-γ-activated Jak2-STAT1 and inflammation. Western blotting was used to determine LC3 conversion (A) and the time kinetic phosphorylation of Jak2 (Tyr¹⁰⁰⁷/Tyr¹⁰⁰⁸) and STAT1α/β (Tyr⁷⁰¹) (B) in IFN-γ (10 ng/ml)-treated WT and Atg7^−/− MEFs. β-Actin was the internal control. Data are representative of three individual experiments. C, Griess reagent was used to detect nitrite generation 48 h after IFN-γ (10 ng/ml) treatment. D, ELISA was used to measure IP-10 production 24 h after IFN-γ (10 ng/ml) treatment. Data, obtained from triplicate cultures, are means ± S.D. (error bars). *, p < 0.05.