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. 2001 Jan 30;98(4):2047–2052. doi: 10.1073/pnas.021449998

Figure 2.

Figure 2

DCMU prevents the starvation-induced increase in transcript levels of nuclear-encoded photosynthetic genes. Samples were prepared as described in Fig. 1. DCMU in ethanol was added at time 0 to a final concentration of 10 μM; controls were treated with ethanol (0.1%). Samples were removed after 24 h and RNA extracted for RNA blot analysis. Autoradiographs of the resulting RNA blots were scanned and signal intensity quantified; the values presented are the averages and standard errors from six independent experiments; the values have been normalized on the constitutively expressed gene (ARF), and are presented relative to the expression level in sucrose-treated cells. An analysis of variance test was performed on the data and significant probability levels given on the graph. (A) CAB; (B) RBCS; (C) NR; (D) chalcone synthase, CHS; (−) and (+), absence and presence of 3% (wt/vol) sucrose; and D, presence of 10 μM DCMU.