FIGURE 3.

Ankyrin-B, Kir6.2, and SUR1/SUR2A associate in a ternary complex. A and B, co-immunoprecipitation experiments demonstrated association of ankyrin-B, Kir6.2, and SUR1/SUR2A. HEK293 cells (which express ankyrin-B, but not Kir6.2, SUR1, or SUR2A; C, first lane) were transfected with Kir6.2 and/or SUR1/SUR2A as indicated. Cell lysates were immunoprecipitated (IP) with either anti-Kir6.2 Ig (A) or anti-ankyrin-B Ig (B), and bound protein was analyzed by SDS-PAGE and immunoblotting (IB) using anti-ankyrin-B, anti-Kir6.2, anti-SUR1, and anti-SUR2A antibodies. Note that Kir6.2 expression was required for ankyrin-B/SUR1 and ankyrin-B/SUR2A association. C, expression control demonstrating that HEK293 cells express transfected proteins.