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Western blot analysis of C1q in carp serum (lane M: molecular marker). Carp serum was electrophoresed on 12% SDS-PAGE gel using Laemmli buffer. The proteins separated were blotted on nitrocellulose membrane and immunostained with rabbit anti-fish ghA, ghB, or ghC antibodies, followed by staining with HRP-labeled anti-rabbit IgG.
