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Hemolytic study of carp serum using SRBC sensitized with carp anti-SRBC antibody. 100 μl of serum was incubated with an equal amount of sensitized SRBC at 37 °C for 30 min. After centrifugation, the sera were tested for hemolytic activity by measuring the A405 values. The guinea pig serum (1:20 diluted) was used as a positive control. Vertical bars represent the mean ± S.D. (n = 4). Significant differences are indicated with asterisks at p < 0.05.
