FIGURE 6.

Inhibition by Trx-ghA, Trx-ghB, or Trx-ghC of C1q-dependent hemolysis of SRBC sensitized with rabbit anti-SRBC antibody (IgG) (A), mouse anti-SRBC antibody (IgM) (B), and carp anti-SRBC antibody (C). In A and B, SRBC sensitized with IgG (SRBC_IgG) or IgM (SRBC_IgM) were pretreated with various concentrations of Trx-ghA, Trx-ghB, or Trx-ghC for 1 h at 37 °C. 1:20 diluted guinea pig serum was then added to pretreated SRBC to initiate hemolysis. In C, nonimmunized zebrafish serum diluted 1:3 was alternatively used; nonimmunized zebrafish serum has been proven previously to not lead to hemolysis). The unlysed cells were centrifuged, and A₄₀₅ values of the supernatants were measured. The percentage inhibited was determined relative to the untreated sample. The means of three experiments are presented for each set of experiments.