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. 2010 Jul 8;285(37):28777–28786. doi: 10.1074/jbc.M110.131318

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Binding of heat-aggregated IgG and IgM to Trx-ghA, Trx-ghB, and Trx-ghC. Various concentrations of Trx-ghA, Trx-ghB, or Trx-ghC were coated to the microtiter wells and a fixed concentration of either heat-aggregated human IgG (10 μg/well) (A) or heat-aggregated human IgM (20 μg/well) (B) was added and probed with HRP-conjugated anti-human IgG (A) or anti-human IgM (B), respectively. Color was developed using p-nitrophenyl phosphate, and A₄₅₀ values were measured. All experiments were performed in duplicate, and Trx was used as a negative control.