FIGURE 1.

Degradation of Nup62 during HRV2 infection. A, schematic representation of Nup62 and regions recognized by anti-Nup62 antibodies. Vertical white bars indicate the location of FG repeats. Nup62(N) and Nup62(C) indicate the regions of Nup62 used to raise antibodies to the N and C terminus, respectively. B, HRV2 infection induces Nup62 degradation. Twenty-five μg of whole cell lysates prepared from mock-infected cells or cells that had been infected with HRV2 for the indicated length of time were analyzed by immunoblotting with Nup62(N) or Nup62(C). Immunoblots were stripped and reprobed to detect nucleolin and eIF4GI. Molecular mass markers are indicated in kilodaltons. % remaining, band intensities were quantitated by densitometry, and the amounts relative to mock-infected cells are indicated. ‡, eIF4GI cleavage products. longer exposure, a longer exposure of the Nup62(N) blot.