FIGURE 5.

Nup62 is cleaved at Ala¹⁰³ in HRV2-infected cells. Plasmids encoding GFP or GFP fused to wild type or mutant Nup62 (GFP-Nup62Wt or GFP-NUP62A103D, respectively) were transfected into HeLa cells, and 24 h later were infected with HRV2. Whole cell lysates prepared 18 h later were electrophoresed, transferred to PVDF membrane, and analyzed by immunoblotting with antibodies recognizing GFP, Nup62(N), eIF4GI, or nucleolin. Molecular mass markers are indicated in kilodaltons. None, cells were treated with transfection reagent without plasmid. Cleavage product arising due to proteolysis at Ala¹⁰³ is indicated with an asterisk.