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Effect of copper/iron on the secondary structure of NEILs. A, NEIL1 plus CuCl2 (0.1 or 0.5 μm). B, NEIL1 plus FeSO4 (0.5 or 1 μm). C, NEIL1 plus MgCl2 (0.1 or 1 μm). D, NEIL2 plus CuCl2 (0.1 or 0.5 μm). The CD spectra were recorded at 195–260 nm, and CD contribution from metal alone was subtracted from metal-protein complex spectra.
