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. 2010 Jun 22;285(37):28826–28837. doi: 10.1074/jbc.M109.099424

FIGURE 2.

FIGURE 2.

Expression of markers related to osteoblasts and osteoclasts in bones and sera in wild-type and CCR1−/− mice. In A, B, and D, total RNAs were isolated form the proximal tibia of wild-type and Ccr1−/− male mice at 8 weeks of age. Real-time Q-PCR revealed the relative expression levels of osteoblast-related mRNAs (Runx-2, Osterix, Atf4, Osteonectin, Osteopontin, Osteocalcin, and Collagen1α1, A), osteoclast-related mRNA (Trap5a and Cathepsin K, B), and RANK–RANKL axis (Rank and Rankl, D). Data are expressed as the copy numbers of these markers normalized to Gapdh expression (mean ± S.E., n = 8). In C, the levels of serum BALP, TRAP, and serum collagen-type1 N-telopeptides (NTx) were measured by ELISA. The bars indicate the mean ± S.E. Each sample was duplicated. Wild-type and Ccr1−/− male mice at 9 weeks of age (n = 10 and 6, respectively) were subjected to BALP and TRAP. Wild-type and Ccr1−/− male mice at 9–13 weeks of age (n = 8 and 6, respectively) were assayed for NTx. #, significantly different from wild-type controls, p < 0.05. N.D., not detected.