FIGURE 7.

CCR1 is involved in the RANK–RANKL axis and induces the impaired osteoclastogenesis. In A, osteoblastic cells were cultured from the bone marrow of wild-type and Ccr1^−/− mice. Relative expression levels of Rankl by Ccr1^−/− osteoblasts as measured by real-time Q-PCR (mean ± S.E., n = 3). ^#, significantly different from wild-type controls, p < 0.05. In B and C, the number of TRAP⁺ multinuclear osteoclasts induced by co-culture with osteoblasts. Co-culture with osteoblastic cells isolated from wild-type or Ccr1^−/− mice (mean ± S.E., duplicated, n = 2, B), and with osteoclast precursors isolated from wild-type or Ccr1^−/− mice (mean ± S.E., duplicated, n = 2, C). Osteoclast cultures with M-CSF and RANKL without osteoblasts were set as positive control. ^#, significantly different from co-culture of osteoclasts with wild-type osteoblasts, p < 0.05.