FIGURE 2.

Genome-wide measurements reveal large differences in translational efficiency under conditions in which the canonical cap-dependent initiation mechanism is presumed to predominate. Translational efficiency is determined by comparing the amount of ribosome-associated mRNA with the total pool of mRNA for each gene. The data are normalized such that the median translational efficiency is equal to 1 (log₂ = 0). Some genes with unusual translational efficiencies are highlighted and discussed in the text. The data are for wild-type yeast grown in rich media (36).