TABLE 1.
Properties of DnaX complexes with various combinations of wild-type and K51E DnaX protomers
| DnaX Complex | No. of active DnaX | β2K0.5 | Specific activity of replicase | ATPase (units/pmol of DnaXcx)a |
|---|---|---|---|---|
| nm | units/mg × 106 | |||
| τ3 | 3 | 1.5 | 4.1 | 520 |
| γ3 | 3 | 1.2 | 96 | 400 |
| τ2γ | 3 | 0.4 | 1.9 | 470 |
| τγ2 | 3 | 1.4 | 24 | 230 |
| τ2γm | 2 | 11 | 1.5 | 21 |
| τmγ2 | 2 | 6.6 | 13 | 7.9 |
| τm2γ | 1 | 15 | 1.0 | 3.3 |
| τγm2 | 1 | 37 | 1.2 | 4.4 |
| τm3 | 0 | NDb | 0.04 | −1.2 |
| γm3 | 0 | NDb | 0.03 | 0.2 |
a Unit = pmol of ATP hydrolyzed/min. Assays represent the average of three or more assays.
b ND, not detectable.