FIGURE 2.

Purification of the five extrinsic proteins from crude PSII of a marine centric diatom, C. gracilis. Lane 1, crude PSII; lane 2, five extrinsic proteins extracted from the crude PSII by alkaline Tris treatment; lane 3, purified PsbO; lane 4, purified PsbQ′; lane 5, purified PsbV; lane 6, purified Psb31; and lane 7, purified PsbU. The gel was stained with Coomassie Brilliant Blue R-250, and each polypeptide was identified by immunological and/or N-terminal sequence analyses according to the methods described previously (29, 30). L, large subunit; S, small subunit; Cyt, cytochrome.