TABLE 5.
Oxygen evolution of the urea/NaCl-treated PSII reconstituted with the extrinsic proteins in various combinations under the Mn-photoactivation condition
| Oxygen evolution (μmol of O2/mg of Chl/h)a |
|||
|---|---|---|---|
| − ions | +10 mm NaCl | +5 mm CaCl2 | |
| Untreated PSII | 2143 ± 123 (100) | 2240 ± 117 (100) | 2240 ± 140 (100) |
| Urea/NaCl-treated PSII | 0 (0) | 90 ± 9 (4) | 134 ± 19 (6) |
| + PsbO | 173 ± 17 (8) | 537 ± 35 (24) | 985 ± 62 (44) |
| + PsbQ′ | 0 (0) | 112 ± 13 (5) | 134 ± 18 (6) |
| + Psb31 | 128 ± 12 (6) | 150 ± 15 (7) | 156 ± 19 (7) |
| + PsbO + PsbQ′ | 186 ± 13 (9) | 542 ± 38 (24) | 1044 ± 78 (47) |
| + PsbO + Psb31 | 169 ± 17 (8) | 588 ± 41 (26) | 979 ± 77 (44) |
| + PsbO + PsbQ′ + Psb31 | 173 ± 24 (8) | 603 ± 40 (27) | 1070 ± 61 (48) |
| + PsbO + PsbQ′ + Psb31 + PsbV | 283 ± 27 (13) | 808 ± 42 (36) | 1012 ± 72 (45) |
| + PsbO + PsbQ′ + PsbV + Psb31 + PsbU | 988 ± 74 (46) | 1113 ± 66 (50) | 1130 ± 90 (50) |
a Values are shown as mean ± S.D., with n = 3 (three independent experiments).