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. 2010 Jul 13;285(38):29223–29230. doi: 10.1074/jbc.M110.144576

FIGURE 1.

FIGURE 1.

Expression of AQP1 and AQP4 in astrocytoma cell line transfected with anti- or pre-miR-320a. A, relative miR-320a expression in astrocytoma cell line transfected with anti- or pre-miR-320a at a concentration of 30 nm. B, relative mRNA expression of AQP1, AQP4 in astrocytoma cell line transfected with anti- or pre-miR-320a at a concentration of 30 nm. Statistical analyses were done using t tests. *, p < 0.05; **, p < 0.01 compared with respective negative controls. The data shown are the means ± S.D., n = 3. C, AQP1 and AQP4 protein expression in astrocytoma cell line transfected with anti- or pre-miR-320a. Changes observed in mRNA expression were reflected in protein expression as well. β-Actin was used as a loading control. D, AQP immunoreactivites in astrocytoma cells transfected with anti- or pre-miR-320a. Human astrocytes were co-transfected with AQP1 or AQP4 plasmids and anti- or pre-miR-320a. The cells were fixed and immunolabeled with either anti-AQP1 or anti-AQP4 antibodies (green) and nuclear stain DAPI (blue). Anti-miR-320a-treated cells showed increased immunoreactivity for AQP1 and AQP4, whereas pre-miR-320a-treated cells showed a reduction in immunoreactivity.