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. 2010 Jul 13;285(38):29223–29230. doi: 10.1074/jbc.M110.144576

FIGURE 4.

FIGURE 4.

Analyses of brain sections of MCA occluded rats injected with anti- or pre-miR-320a. A, expression level of miR-320a in ischemic rat injected with anti- or pre-miR-320a. Changes in miR-320a expression levels in the brain samples were quantitated using stem-loop real time quantitative PCR. B, histological analysis of brain sections. 2,3,5-Triphenyltetrazolium chloride-stained coronal brain sections (2 mm thick) of rats injected with 50 pmol of anti- or pre-miR-320a. Intracerebroventricular injections were given immediately after the removal of the suture (n = 10). Surviving cells stained red, whereas dead cells remained white. C, infarct volume of rat treated with anti- or pre-miR-320a. Infarct volumes are expressed as percentages of the control ± S.E. D, AQP1 and AQP4 mRNA levels in rats injected with anti- or pre-miR-320a. The data shown are the means ± S.D., n = 10. mRNA expression correlated with protein expression as well (see inset). E, relative AQP1, AQP4, and miR-320a expression in MK-801 administered ischemic (MCAo) rats. The rats with MCA occlusion were injected with MK-801 to reduce cerebral infarct. All of the values were expressed relative to ischemic rats. Statistical analyses were done using t tests. **, p < 0.01. The data shown are the means ± S.D., n = 10. Ctrl, control.