FIGURE 3.

Activity of CC-CXCL8 in neutrophils. Panel A, dose-response of intracellular Ca²⁺ release in neutrophils. Human neutrophils loaded with Indo-1 were stimulated with different CC-CXCL8 concentrations (10–1000 nm) and the intracellular Ca²⁺ release was measured by fluorescence measurements. Panels B–G, effect of CXCR2 inhibitor SB225002. Human neutrophils loaded with Indo-1 were tested for Ca²⁺ release in the absence and presence of SB225002 for 100 nm CXCL8 (panels B and C), 100 nm CXCL1 (panels D and E), and 200 nm CC-CXCL8 (panels F and G).