FIGURE 4.
Activity of CC-CXCL8 in HL-60 cells individually expressing CXCR1 and CXCR2. Panel A, HL-60 cells expressing CXCR1 loaded with Indo-1 were stimulated with CXCL8 or CC-CXCL8 and the Ca2+ release was monitored by fluorescence spectroscopy. Panels B and C, HL-60 cells expressing CXCR2 were loaded with Indo-1 and stimulated with 100 nm CXCL8 or 200 nm CC-CXCL8. Panel D, neutrophil recruitment in a mouse lung model. BAL samples from mice treated with CC-CXCL8, WT CXCL8, or PBS control were collected 6 h post-inoculation. Each data set represents average over 4–6 animals/group.