Skip to main content
. 2010 Jul 14;285(38):29262–29269. doi: 10.1074/jbc.M110.146555

FIGURE 5.

FIGURE 5.

NMR structural characteristics of CC-CXCL8 dimer. Panel A, intramolecular NOEs from Phe-17 NH to Trp-57 side chain protons, and (panels B and C) intermolecular NOEs across the dimer interface from Val-27 to Leu-25′ and Glu-24′ and from Phe-65 to Val-27′ and Glu-29′ are shown (residues across the dimer interface are highlighted by the sign ‘). Panel D shows the proximity of these residues in the WT CXCL8 structure (PDB ID: 1IL8); tertiary interactions within the monomer unit are shown in the top panel, and the quaternary interactions across the dimer interface are shown in the bottom panel. Panel E, 1H-15N HSQC spectrum of the CC-CXCL8 dimer. The chemical shifts are similar to those observed for the WT CXCL8. Characteristic upfield- and downfield-shifted peaks are labeled. Diagonal plot of HN (panel F) and Hα (panel G) chemical shifts of CC-CXCL8 (y-axis) and WT CXCL8 (x-axis).