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Sedimentation equilibrium studies of WT CXCL8 and CC-CXCL8
| Protein | Buffer | Kd (μma) |
|---|---|---|
| WT CXCL8 | 50 mm phosphate, pH 7 | 12 ± 5b |
| 50 mm acetate, pH 5.0 | 15 ± 3 | |
| CC-CXCL8 | 50 mm phosphate, pH 7 | 6 ± 2 |
| 50 mm acetate, pH 5.0 | 9 ± 3 |
a The Kd was calculated from a global fit of the data collected at rotor speed of 25,000, 30,000, and 42, 000 rpm. The phosphate buffer also contains 50 mm NaCl.
b Data from Ref. 16.
