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EMSAs with purified RosR protein and DNA fragments (80 ng each) covering the promoter regions of narKGHJI, cg1426, and cg2329. A DNA fragment containing parts of the coding regions of narK and narG was used as the negative control. The DNA fragments were incubated either without RosR or with the indicated concentrations of RosR for 20 min at room temperature. Then the samples were separated by native polyacrylamide gel electrophoresis (10%), and the DNA was stained with GelRedTM.
