FIGURE 3.
EMSAs with purified RosR protein and the promoter regions of putative target genes predicted from an in silico search. DNA fragments (80 ng) covering the promoter regions of cg3237 (superoxide dismutase), cg1150 (putative FMN reductase), cg1426 (putative protein of the glutathione S-transferase family), cg2329 (putative monooxygenase), cg1848 (putative monooxygenase), rosR, and cg3084 (putative monooxygenase) were incubated for 20 min at room temperature with the given concentrations of purified RosR. The negative control contained the narK promoter without the predicted RosR binding site. Samples were separated by native PAGE (10%) and stained with GelRedTM.