TABLE 1.
Urea-induced changes in liver mass in perfused rat liver
Rat livers were perfused as described under “Experimental Procedures.” Data are given as mean ± S.E. of the mean; n refers to the number of independent perfusion experiments. When indicated, integrin-antagonistic GRGDSP peptide (10 mmol/liter), nonantagonistic GRGESP control peptide (10 mmol/liter), PP-2 (250 nmol/liter), or SB203580 (100 nmol/liter) were instituted 30 min prior to the infusion of urea or the respective urea derivates. Liver mass was continuously monitored and maximal change in liver mass, which was reached within 303 ± 15 s (n = 77), was determined.
| Condition | Change in liver mass | n |
|---|---|---|
| % of total liver mass | ||
| Control | ±0.0 ± 0.0 | 3 |
| Urea (5 mm) | ±0.0 ± 0.0 | 3 |
| Urea (100 mm) | −4.6 ± 0.5 | 9 |
| +GRGDSP | −4.0 ± 0.9 | 3 |
| +GRGESP | −5.1 ± 0.2 | 3 |
| +PP2 | −2.7 ± 0.3a | 3 |
| +SB203580 | −1.6 ± 0.8a | 3 |
| Urea (200 mm) | −5.4 ± 0.5 | 11 |
| +SB203580 | −2.6 ± 0.4a | 3 |
| Methylurea (100 mm) | −1.9 ± 0.2 | 3 |
| Dimethylurea (100 mm) | −2.1 ± 0.6 | 3 |
| Thiourea (10 mm) | −1.8 ± 0.3 | 3 |
| Thiourea (50 mm) | −4.4 ± 0.9 | 5 |
| +SB203580 | −1.3 ± 0.8a | 3 |
| Thiourea (100 mm) | −5.6 ± 1.6 | 4 |
| Thiourea (200 mm) | −6.2 ± 0.6 | 3 |
| Dimethylthiourea (100 mm) | −5.1 ± 0.8 | 4 |
| Hypo-osmolarity (−30 mm NaCl) | +10.0 ± 1.3 | 4 |
| Hypo-osmolarity (−40 mm NaCl) | +12.6 ± 0.8 | 4 |
a The asterisk indicates a significant inhibition of urea-induced change in liver mass by the respective inhibitor (p < 0.05).