TABLE 1.

N-Glycan microheterogeneity of renal GGT as determined by LC/MS-MS

Relative abundance values were deduced from comparative ion intensities among glycopeptide family members. The tryptic peptides in which each glycosylation site is contained are depicted in Fig. 3. Calculated m/z values for the corresponding permethylated N-glycans enzymatically released from either renal GGT (a) or from the pool of kidney membrane glycoproteins (b) in Fig. 2 are included for reference. Abbreviations used are as follows: Hex, hexose (mannose and galactose); HexNac, N-acetylglucosamine; dHex, deoxyhexose (fucose); NeuAc, neuraminic (sialic) acid; ND, not detected.