FIGURE 4.

Regulation of LRRK2 autophosphorylation activity by the three possible phosphorylation sites (Thr-2031, Ser-2032, and Thr-2035). A and C, immunoblot and autoradiogram of autophosphorylated LRRK2, as resolved by SDS-PAGE. LRRK2 protein levels were determined by Western blot analysis, using anti-myc antibody. B and D, normalization of incorporated ³²P compared with LRRK2 protein content. Data represent three independent experiments, in arbitrary units, where wild type (WT)-LRRK2 kinase activity is defined as 100%. Control bar represents mean ± S.E. *, p < 0.05; **, p < 0.01 compared with wild type LRRK2 kinase activity, assessed by a two-tailed one-sample Student's t test.