Figure 5.

Cloning of GUN5/CCH. (A) Partial amino acid sequence alignment of ChlH around the mutation site of (a) cch and (b) gun5. The substituted residues in the mutants are indicated above the aligned sequences. The derived amino acid sequences of Arabidopsis thaliana ChlH, Nicotiana tabacum ChlH, Antirrhinum majus ChlH, Hordeum vulgare ChlH, Synechocystis sp. PCC6803 ChlH, and Rhodobacter capsulatus BchH are shown. Conserved residues in six ChlH/BchH and in the ChlH counterparts of Co-chelatase CobN from Paracoccus denitrificans, putative Co-chelatase from Methanococcus jannaschii, and Ni-chelatase from Synechocystis PCC 6803 are marked by asterisks. (B) The wild-type CHLH gene rescues gun5. Lhcb mRNA levels were measured as described in Fig. 1B from two representative gun5 lines (#16 and #50) that were transformed with pZG-CHLH.