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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Neuron. 2010 Jul 15;67(1):33–48. doi: 10.1016/j.neuron.2010.06.002

Figure 8. Phosphorylation of Dixdc1 regulates the morphology and structural integrity of neurons.

Figure 8

(A) GFP-positive cells lacking Dixdc1 or expressing Dixdc1 Ser250Ala display abnormal morphology in vivo. Left, images of GFP-positive cells taken from the intermediate zone of electroporated brains in the various conditions indicated. Note that either GFP-positive cells lacking Dixdc1 or expressing the Dixdc1 Ser250Ala mutant show a multipolar morphology, while in control or WT-Dixdc1 conditions, cells have adopted the bipolar morphology that is required for migration into the cortex. Right, graph showing quantification of the percentage of GFP cells that are either multipolar or unipolar/bipolar for the various conditions. (n=250 cells from 4 brains for each condition, **p<0.005, NS=not significant, mean±SEM). The scale bar represents 20 μm. (B) Phosphorylation of Dixdc1 regulates the actin and microtubule cytoskeletal network in neurons. Images of GFP-positive cells cultured from electroporated brains for 72 hours and stained with the indicated markers (phalloidin to label filamentous actin and tubulin for microtubules). Arrows indicate the decreased staining of both markers in GFP-cells lacking Dixdc1 or expressing Dixdc1 Ser250Ala compared to non-electroporated cells. The scale bar represents 20 μm.