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. Author manuscript; available in PMC: 2010 Sep 13.
Published in final edited form as: Angiogenesis. 2008 Nov 27;11(4):395–401. doi: 10.1007/s10456-008-9122-4

Figure 2.

Figure 2

ANG9-4 inhibits Ang1-mediated Tie2 phosphorylation. HEK-293 cells expressing Tie2 were stimulated with Ang1 (14 nM) in the absence or presence of the indicated molar excess of ANG9-4 (9-4) or a scrambled control aptamer (scr), and cell lysates were Western blotted sequentially with anti-phospho-Tie2 (pTie2) and anti-Tie2 as a loading control. (A-C) Progressively lower concentrations of ANG9-4 or control aptamer were tested, as indicated. In (C), cells were also treated with Ang2 (14 nM) to determine effects of ANG9-4 on Tie2 phosphorylation.