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. Author manuscript; available in PMC: 2010 Sep 13.
Published in final edited form as: Liver Transpl. 2009 Sep;15(9):1101–1109. doi: 10.1002/lt.21782

Figure 6.

Figure 6

Cytokine mRNA levels are altered because of TLR4 deficiency. Real-time reverse-transcriptase polymerase chain reaction was used to compare cytokine mRNA levels between groups, and the results are expressed as the fold change over the baseline. In the proinflammatory T-helper 1 cytokines, there were significant increases in IL-12, IFN-γ, and IL-6 mRNA levels at 1 hour of reperfusion in the control HFD animals versus their TLR4KO counterparts (*P < 0.05, n = 5–8/group). There was also a blunting of anti-inflammatory T-helper 2 cytokine production, as evidenced by the increased levels of IL-10 present in the ND control animals, which were dramatically decreased in the HFD control animals (*P < 0.05, n = 5–8/group). Abbreviations: HFD, high-fat diet; IFN-γ, interferon gamma; IL, interleukin; mRNA, messenger RNA; ND, normal diet; TLR4, toll-like receptor 4; TLR4KO, toll-like receptor 4–deficient.