Fig. 6.

Illustration of IgG1 areas affected upon interacting with FcγRIIIa. A space-filling representation (left) and a schematic form (right) are shown in each panel. The Protein Data Bank coordinates used to generate the models are the same as those described in Fig. 2. A, the location of FcγRIIIa interaction on the structure of IgG1. Regions of FcγRIIIa receptor contact with the IgG1 are colored green based on Refs. 32, 43. B, areas where deuterium exchange was altered when the IgG1 became hypergalactosylated. The residues colored red (243–247) correspond to those described in Fig. 2. C, areas where deuterium exchange was altered when FcγRIIIa bound to native IgG1. Changes were observed in the regions colored blue and red as described in Fig. 4. D, areas where deuterium exchange was altered when afucosylated-hypergalactosylated IgG1 bound to FcγRIIIa. Changes were observed in the regions colored yellow and red as described in Fig. 5.